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Sample GSM5121291 Query DataSets for GSM5121291
Status Public on Jul 06, 2021
Title RB14 (expression)
Sample type RNA
Source name retinoblastoma sample RB14
Organism Homo sapiens
Characteristics geo dataset serie: SAMPLE 12
batch: RB01
tissue: retinoblastoma
Growth protocol Immediately following enucleation, a needle was inserted through the anterior chamber of the eye to extract a tumor sample by aspiration. The tumor specimen was placed in RPMI medium on ice, cells were re-suspended, counted and separated into two tubes (for DNA and RNA preparation). The tubes were then centrifuged to remove all media and snap frozen for later extraction.
Extracted molecule total RNA
Extraction protocol Tumor RNA was extracted using Qiazol (Qiagen, Courtaboeuf, France) according to the manufacturer instructions and purified using miRNeasy Mini kit (Qiagen).
Label biotin
Label protocol 100ng of total RNA were amplified to produced biotinylated complementary RNAs, as recommended by Affymetrix.
Hybridization protocol Affymetrix microarrays were hybridized with 10µg of biotinylated cRNA according the supplier recommendations. Microarrays were washed and stained using FS450 fluidics upon a protocol provided by Affymetrix, corresponding to the U133plus 2.0 arrays
Scan protocol Microarrays were scanned using a 7G, Affymetrix GCS3000 scanner, raw data were acquired with Affymetrix Genechip Command Console.
Description Gene expression data from retinoblastoma samples
Data processing Signal reconstruction was performed with the RMA algorithm as implemented in Bioconductor affy package on R statistical software using the Dai custom CDF package: HGU133Plus2_Hs_ENTREZG, Brainarray Custom CDF EntrezG version 23, Batch effects were corrected with the help of the bioconductor package SVA,
Submission date Mar 01, 2021
Last update date Jul 06, 2021
Contact name Elodie chapeaublanc
Organization name Institut Curie
Department UMR 144
Lab Equipe Radvanyi
Street address 26 rue d'ulm
City paris
ZIP/Postal code 75248
Country France
Platform ID GPL28718
Series (2)
GSE58780 Retinoblastoma gene expression data
GSE58785 Pan-genomic study of primary human retinoblastoma samples

Data table header descriptions
VALUE RMA normalized, Brainarray annotated expression values, logscale

Data table
100009676_at 3.26916017874149
10000_at 5.51355154081385
10001_at 7.86906991740576
10002_at 4.27467248047311
100038246_at 3.63905407477283
10003_at 6.29647897636583
100048912_at 2.64660075897253
100049716_at 3.99763555169097
10004_at 3.83234868840816
10005_at 6.17884693330487
10006_at 7.43964189125833
10007_at 7.64590450601585
10008_at 4.02490648015524
100093630_at 9.30884269330613
100093698_at 2.54287430064572
10009_at 6.98919592477128
1000_at 8.59155116674196
100101467_at 5.90166879682453
100101938_at 3.04023476918933
10010_at 6.7567981571462

Total number of rows: 20408

Table truncated, full table size 521 Kbytes.

Supplementary file Size Download File type/resource
GSM5121291_RB01_ARN0006_s1h1s1_U133p2.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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