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Status |
Public on May 18, 2010 |
Title |
BRI1, biological rep1 (expt 1) |
Sample type |
RNA |
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Source name |
35S::BRI1
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Organism |
Arabidopsis thaliana |
Characteristics |
ecotype: Col-0 tissue: vegetative part age: stage 1.03 genotype: pBRI1::BRI1
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Growth protocol |
plant were grown on MS medium under 16 h light/ 8 h dark cycle at 22°C
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Extracted molecule |
total RNA |
Extraction protocol |
RNeasy plant mini kit (Qiagen) , according to manufacturer protocol
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Label |
Standard affymetrix protocol
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Label protocol |
Standard affymetrix protocol
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Hybridization protocol |
Standard affymetrix protocol
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Scan protocol |
Standard affymetrix protocol
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Description |
pBRI1::BRI1, vegetative part of seedlings at stage 1.03
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Data processing |
Expression data were processed using RMA (background correction, normalization and summarization) in BioConductor (Gentleman et al. 2004; Irizarry et al. 2003a; 2003b). An alternative CDF (tinesath1cdf) was used, where each probe is assigned uniquely to one transcript and is excluded if it aligns to a different transcript with 24 or more perfect matches (Casneuf et al. 2007; http://www.bioconductor.org/packages/release/data/experiment/html/tinesath1cdf.html)
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Submission date |
Feb 22, 2010 |
Last update date |
May 18, 2010 |
Contact name |
Nathalie Gonzalez |
E-mail(s) |
nagon@psb.vib-ugent.be
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Phone |
+32 (0)9 331 39 56
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Organization name |
VIB
|
Department |
Plant System Biology
|
Lab |
System Biology of Yield
|
Street address |
Technologiepark 927
|
City |
Gent |
ZIP/Postal code |
9052 |
Country |
Belgium |
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Platform ID |
GPL8455 |
Series (2) |
GSE20455 |
Increased leaf size: different means to an end (experiment 1) |
GSE20458 |
Increased leaf size: different means to an end |
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