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Sample GSM513250 Query DataSets for GSM513250
Status Public on Feb 25, 2010
Title 0 Dis V batch RNA
Sample type RNA
 
Channel 1
Source name wt
Organism Saccharomyces cerevisiae
Characteristics strain name: wt
Biomaterial provider Eduardo Torres, Amon Lab, MIT
Growth protocol Media : minus His plus G418
Extracted molecule total RNA
Extraction protocol Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
Label Cy3
Label protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
 
Channel 2
Source name Dis V
Organism Saccharomyces cerevisiae
Characteristics strain name: Dis V
Biomaterial provider Eduardo Torres, Amon Lab, MIT
Growth protocol Media : minus His plus G418
Extracted molecule total RNA
Extraction protocol Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
Label Cy5
Label protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
 
 
Hybridization protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
Scan protocol Pixel Size : 5
Scan Date : 2009-06-19
Scan Time : 09:27:51
Scanner Make : Agilent Technologies Scanner
Scanner Model : G2505B
Scanning software : ChipScan
Scanning software version : A.7.0.1
Description Detailed protocols are available in 'GSE20464_protocols.zip', which is linked to the Series GSE20464 record as a supplementary file.
0 Dis V batch RNA
Data processing Extraction Software : Agilent Feature Extractor
Extraction Software Version : 9.1.3.1
Datafile type : Agilent result file
Normalizaton: Default settings of Agilent Feature Extractor
 
Submission date Feb 22, 2010
Last update date Feb 24, 2010
Contact name Maitreya J. Dunham
E-mail(s) maitreya@uw.edu
Phone 206-543-2338
Organization name University of Washington
Department Genome Sciences
Lab Dunham Lab
Street address Foege Building, S403B, Box 355065
City Seattle
State/province WA
ZIP/Postal code 98195-5065
Country USA
 
Platform ID GPL10045
Series (1)
GSE20464 Identification of Aneuploidy-Tolerating Mutations

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (R_PROCESSED_SIGNAL/G_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 -.43 267.1155 359.9628 1409.838 362.5946
2 -1.264 17.5614 42.16179 180.1471 103.1471
3 0 1.008443 2.947224 95.71795 67.64103
4 -.456 4766.744 6537.771 23579.83 5739.722
5 -.414 3110.419 4144.525 15418.86 3724.722
6 .251 4617.834 3880.465 22846 3550.971
7 -.414 3656.95 4871.748 18111.57 4510.057
8 -.175 13205.92 14906.16 65160.68 13847
10 -.643 173.4797 270.9299 948.6053 326.3947
11 .852 18.36547 10.17306 184.4722 78.94444
12 -.34 1973.655 2497.71 9818.541 2496.432
13 .049 557.1958 538.6528 2839.593 598.3704
14 1.363 43.88055 17.05534 310.5556 86.25
15 -.45 212.4753 290.2114 1141.378 359.7297
16 .162 10285.44 9194.358 50772.27 9343
17 -.497 3377.445 4767.462 16735.95 4915.132
18 -.38 151.3896 197.0761 840.9714 271.1714
19 -.133 4409.489 4834.836 21821.32 5044.412
20 .401 20.55349 15.568 196.6571 85.68571
21 -.282 2128.953 2588.592 10585.14 2759.111

Total number of rows: 13048

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM513250.txt.gz 4.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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