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Sample GSM513264 Query DataSets for GSM513264
Status Public on Feb 25, 2010
Title 0 Dis XVI-14.2 batch RNA
Sample type RNA
 
Channel 1
Source name disome
Organism Saccharomyces cerevisiae
Characteristics strain name: wt
Biomaterial provider Eduardo Torres, Amon Lab, MIT
Growth protocol Media : minus His plus G418
Extracted molecule total RNA
Extraction protocol Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
Label Cy3
Label protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
 
Channel 2
Source name wt
Organism Saccharomyces cerevisiae
Characteristics strain name: Dis XVI-14.2
Biomaterial provider Eduardo Torres, Amon Lab, MIT
Growth protocol Media : minus His plus G418
Extracted molecule total RNA
Extraction protocol Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
Label Cy5
Label protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
 
 
Hybridization protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
Scan protocol Pixel Size : 5
Scan Date : 2009-06-26
Scan Time : 08:32:41
Scanner Make : Agilent Technologies Scanner
Scanner Model : G2505B
Scanning software : ChipScan
Scanning software version : A.7.0.1
Description Detailed protocols are available in 'GSE20464_protocols.zip', which is linked to the Series GSE20464 record as a supplementary file.
0 Dis XVI-14.2 batch RNA
Data processing Extraction Software : Agilent Feature Extractor
Extraction Software Version : 9.1.3.1
Datafile type : Agilent result file
Normalizaton: Default settings of Agilent Feature Extractor
 
Submission date Feb 22, 2010
Last update date Feb 24, 2010
Contact name Maitreya J. Dunham
E-mail(s) maitreya@uw.edu
Phone 206-543-2338
Organization name University of Washington
Department Genome Sciences
Lab Dunham Lab
Street address Foege Building, S403B, Box 355065
City Seattle
State/province WA
ZIP/Postal code 98195-5065
Country USA
 
Platform ID GPL10045
Series (1)
GSE20464 Identification of Aneuploidy-Tolerating Mutations

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (R_PROCESSED_SIGNAL/G_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 .124 48.52946 44.54322 138.8649 81.02703
2 -1.48 2.301737 6.420248 86.86111 61.72222
3 -.685 3.700981 5.951874 94.23077 61.53846
4 -.259 1157.163 1384.827 1234.297 750.5946
5 .476 3525.27 2535.302 3566.154 1319.179
6 -1.499 4268.966 12066.36 4289.091 6027.909
7 .256 3808.794 3190.591 3825.875 1627.95
8 .261 12661.2 10565.53 12477.05 5228.974
10 .192 144.3076 126.3491 231.4118 120.1765
11 .344 51.21178 40.34479 140.7368 78.55263
12 1.305 3564.299 1442.223 3541.459 749.2703
13 -.815 275.5984 484.9231 357.2857 289.9143
14 -.87 13.69324 25.02286 104.4474 71.07895
15 .301 302.7951 245.6925 382.359 174.9487
16 -.128 14094.5 15400.84 13607.85 7270.78
17 -.077 2996.968 3161.632 2959.718 1532.487
18 .151 156.3858 140.821 240.9474 124.8158
19 .17 2940.103 2613.18 2895.576 1265.939
20 -.15 140.2766 155.7003 225.2105 131.1316
21 .11 717.9009 664.968 774.0313 363.7813

Total number of rows: 13048

Table truncated, full table size 584 Kbytes.




Supplementary file Size Download File type/resource
GSM513264.txt.gz 4.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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