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Sample GSM513273 Query DataSets for GSM513273
Status Public on Feb 25, 2010
Title AA Evolved Disome 9.2 c1 P-lim Chemostat RNA 4x44k
Sample type RNA
 
Channel 1
Source name F1 12/6/05 RNA
Organism Saccharomyces cerevisiae
Characteristics strain name: wt
Biomaterial provider Cheryl Christianson, Dunham Lab, Princeton University
Growth protocol Media : phosphate-limited chemostat media
Extracted molecule total RNA
Extraction protocol Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
Label Cy3
Label protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
 
Channel 2
Source name F20 4/17/07 RNA
Organism Saccharomyces cerevisiae
Characteristics strain name: Evolved Disome IX 14.2
Biomaterial provider Cheryl Christianson, Dunham Lab, Princeton University
Growth protocol Media : phosphate-limited chemostat media
Extracted molecule total RNA
Extraction protocol Extraction method: acid phenol prep:
http://dunham.gs.washington.edu/MDyeastRNA.pdf
Label Cy5
Label protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
 
 
Hybridization protocol reference URL:
http://dunham.gs.washington.edu/MDAgilentRNA.pdf
Scan protocol Pixel Size : 5
Scan Date : 2007-05-25
Scan Time : 11:19:10
Scanner Make : Agilent Technologies Scanner
Scanner Model : G2505B
Scanning software : ChipScan
Scanning software version : A.7.0.1
Description Detailed protocols are available in 'GSE20464_protocols.zip', which is linked to the Series GSE20464 record as a supplementary file.
AA Evolved Disome 9.2 c1 P-lim Chemostat RNA 4x44k
Data processing Extraction Software : Agilent Feature Extractor
Extraction Software Version : 9.5.1.1
Datafile type : Agilent result file
Normalizaton: Default settings of Agilent Feature Extractor
 
Submission date Feb 22, 2010
Last update date Feb 24, 2010
Contact name Maitreya J. Dunham
E-mail(s) maitreya@uw.edu
Phone 206-543-2338
Organization name University of Washington
Department Genome Sciences
Lab Dunham Lab
Street address Foege Building, S403B, Box 355065
City Seattle
State/province WA
ZIP/Postal code 98195-5065
Country USA
 
Platform ID GPL7542
Series (1)
GSE20464 Identification of Aneuploidy-Tolerating Mutations

Data table header descriptions
ID_REF Uniquely identifies feature/spot in array layout.
VALUE log(base 2) (R_PROCESSED_SIGNAL/G_PROCESSED_SIGNAL)
R_PROCESSED_SIGNAL The propagated feature signal in the red channel, used for computation of log ratio
G_PROCESSED_SIGNAL The propagated feature signal in the green channel, used for computation of log ratio
R_MEAN_SIGNAL Mean foreground intensity Ch 2.
G_MEAN_SIGNAL Mean foreground intensity Ch 1.

Data table
ID_REF VALUE R_PROCESSED_SIGNAL G_PROCESSED_SIGNAL R_MEAN_SIGNAL G_MEAN_SIGNAL
1 -.135 1451.221 1593.12 541.2295 221.0492
2 0 5.533627 10.44294 22.80328 20.40984
3 -1.035 5.497224 11.26771 21.6875 22
4 0 5.458215 10.23749 19.85 21.9
5 -1.95 5.443464 21.02809 20.18333 23.23333
6 -1.699 5.403976 17.54918 20.18644 22.88136
7 0 5.355597 9.961697 22.66129 21.53226
8 0 5.323504 9.876241 22.03175 21.26984
9 0 5.294498 9.798624 21.21667 20.78333
10 -1.58 5.278919 15.78709 20.76812 22.86957
11 0 5.238313 9.650258 21.19643 21
12 -.516 61.45575 87.87344 48.88333 32.13333
13 -.078 2020.106 2132.144 793.9559 320.2353
14 .323 3956.722 3164.09 1529.569 470.9077
15 .101 4692.06 4375.376 1814.091 647.8333
16 -.079 612.7572 647.3866 267.8 110.6769
17 -1.067 662.9246 1389.282 284.5397 217.4762
18 .831 1153.911 648.7641 481.0164 112.9836
19 -.166 355.2186 398.6259 169.5781 76.01563
20 -.849 18.46521 33.26227 31.73529 25.52941

Total number of rows: 45209

Table truncated, full table size 2045 Kbytes.




Supplementary file Size Download File type/resource
GSM513273.txt.gz 12.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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