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Sample GSM5133673 Query DataSets for GSM5133673
Status Public on Mar 02, 2024
Title Serum-NegativeControl-rep7-Phase I
Sample type protein
 
Channel 1
Source name Serum,NegativeControl,Phase I
Organism Homo sapiens
Characteristics gender: female
age: 59
sampletype: Serum
cancertype: normal
designphase: Phase I
molecule: Serum autoantibody proteins IgG
Extracted molecule protein
Extraction protocol standard protocol
Label Cy3
Label protocol After washing with PBST, the microarray was incubated with 1:1000 diluted Fluor conjugated goat anti-human IgG (532nm) and donkey anti-human IgM (635nm) (Jackson ImmunoResearch, West Grove, PA) at RT for 1h in the dark. After washing with PBST, the microarray was rinsed with dd H2O and dried
 
Channel 2
Source name Serum,NegativeControl,Phase I
Organism Homo sapiens
Characteristics molecule: Serum autoantibody proteins IgM
Extracted molecule protein
Extraction protocol standard protocol
Label Cy5
Label protocol After washing with PBST, the microarray was incubated with 1:1000 diluted Fluor conjugated goat anti-human IgG (532nm) and donkey anti-human IgM (635nm) (Jackson ImmunoResearch, West Grove, PA) at RT for 1h in the dark. After washing with PBST, the microarray was rinsed with dd H2O and dried
 
 
Hybridization protocol The microarray was taken out from − 80°C and then incu-bated in blocking buffer (3% BSA in PBS) at RT for 3h.Then a serum sample diluted at 1:200 in binding buffer (1%BSA in PBST) was added to the microarray and incubatedat 4°C overnight
Scan protocol The micro-array was scanned with the LuxscanTM 10K-A (CapitalBio Corporation, Beijing, China).
Description Phase I of the experimental design
Data processing Data analysis was carried out using The GenePix Pro 6.0 (Axon Instruments, Foster City, CA). The signal for each spot (SNR) was defined as the ratio of the fore-ground to the background median intensity and normalized using the median value.
 
Submission date Mar 03, 2021
Last update date Mar 02, 2024
Contact name He Yan Bin
E-mail(s) heyanbin@bcbio.cn
Phone 18565610792
Organization name BCBIO
Street address Sino-European Center A, 2 Lingnan Avenue South, Shunde, Foshan, Guangdong, China
City Foshan
State/province GUANGDONG
ZIP/Postal code 528315
Country China
 
Platform ID GPL29809
Series (1)
GSE168198 Human serum: Control vs. Lung Cancer

Data table header descriptions
ID_REF
VALUE Median value normalized of SNR( SNR is the ratio of foreground to the background median intensity of each spot).

Data table
ID_REF VALUE
1 0.890331612
2 2.7585673
3 0.996143358
4 1.832784134
5 0.658941573
6 1.695343683
7 0.926444512
8 1.035927371
9 0.586606658
10 1.851864757
11 1.551100611
12 0.690194922
13 0.347104531
14 0.369536738
15 0.368294163
16 0.892199744
17 0.971450937
18 2.471170792
19 0.344984879
20 0.379691907

Total number of rows: 46066

Table truncated, full table size 793 Kbytes.




Supplementary file Size Download File type/resource
GSM5133673_20180316-9006329-NC7-R600G500.gpr.gz 4.5 Mb (ftp)(http) GPR
Processed data included within Sample table

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