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Sample GSM5133747 Query DataSets for GSM5133747
Status Public on Mar 02, 2024
Title Serum-LungCancer-rep5-Phase II
Sample type protein
 
Channel 1
Source name Serum,LungCancer,Phase II
Organism Homo sapiens
Characteristics gender: female
age: 55
sampletype: Serum
cancertype: LungCancer
designphase: Phase II
molecule: Serum autoantibody proteins IgG
Extracted molecule protein
Extraction protocol standard protocol
Label Cy3
Label protocol After washing with PBST, the microarray was incubated with 1:1000 diluted Fluor conjugated goat anti-human IgG (532nm) and donkey anti-human IgM (635nm) (Jackson ImmunoResearch, West Grove, PA) at RT for 1h in the dark. After washing with PBST, the microarray was rinsed with dd H2O and dried
 
Channel 2
Source name Serum,LungCancer,Phase II
Organism Homo sapiens
Characteristics molecule: Serum autoantibody proteins IgM
Extracted molecule protein
Extraction protocol standard protocol
Label Cy5
Label protocol After washing with PBST, the microarray was incubated with 1:1000 diluted Fluor conjugated goat anti-human IgG (532nm) and donkey anti-human IgM (635nm) (Jackson ImmunoResearch, West Grove, PA) at RT for 1h in the dark. After washing with PBST, the microarray was rinsed with dd H2O and dried
 
 
Hybridization protocol The microarray was taken out from − 80°C and then incu-bated in blocking buffer (3% BSA in PBS) at RT for 3h.Then a serum sample diluted at 1:200 in binding buffer (1%BSA in PBST) was added to the microarray and incubatedat 4°C overnight
Scan protocol The micro-array was scanned with the LuxscanTM 10K-A (CapitalBio Corporation, Beijing, China).
Description Phase II of the experimental design
Data processing Data analysis was carried out using The GenePix Pro 6.0 (Axon Instruments, Foster City, CA). The signal for each spot (SNR) was defined as the ratio of the fore-ground to the background median intensity and normalized using the median value.
 
Submission date Mar 03, 2021
Last update date Mar 02, 2024
Contact name He Yan Bin
E-mail(s) heyanbin@bcbio.cn
Phone 18565610792
Organization name BCBIO
Street address Sino-European Center A, 2 Lingnan Avenue South, Shunde, Foshan, Guangdong, China
City Foshan
State/province GUANGDONG
ZIP/Postal code 528315
Country China
 
Platform ID GPL29810
Series (1)
GSE168198 Human serum: Control vs. Lung Cancer

Data table header descriptions
ID_REF
VALUE Median value normalized of SNR( SNR is the ratio of foreground to the background median intensity of each spot).

Data table
ID_REF VALUE
1 12.05548731
2 4.167738971
3 15.12658228
4 14.02739726
5 6.814608135
6 11.01172161
7 3.754509804
8 10.7752381
9 58.39077085
10 13.25974026
11 121.4077731
12 31.31038961
13 18.23696586
14 15.1047619
15 8.522108549
16 63.26470588
17 10.7343985
18 21.83198648
19 11.99278499
20 20.70357143

Total number of rows: 182

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM5133747_Phase2_LC_121.gpr.gz 25.5 Kb (ftp)(http) GPR
Processed data included within Sample table

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