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| Status |
Public on Mar 12, 2021 |
| Title |
ffibro |
| Sample type |
SRA |
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| Source name |
Fibroblast
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| Organism |
Mustela putorius furo |
| Characteristics |
cell type: Fibroblast
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| Treatment protocol |
See the citation
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| Growth protocol |
See the citation
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
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| Description |
ffibro
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| Data processing |
Data of RNA-seq (fastq file format) were quality-checked, and low-quality reads (score < 30), adapter sequences, and overrepresented sequences such as poly-A chain were trimmed using the Trim Galore! (ver.0.4.0).
The remaining reads were mapped to the human genome (hg19) using the STAR (ver.2.5.3a), and the output file (BAM file format) were summarized using the featureCounts (1.5.2).
Genome_build: hg19 (Human genome)
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
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| Submission date |
Mar 11, 2021 |
| Last update date |
Mar 12, 2021 |
| Contact name |
Sho Yoshimatsu |
| E-mail(s) |
yoshima@a7.keio.jp
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| Organization name |
Keio University School of Medicine
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| Department |
Physiology
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| Lab |
Hideyuki Okano's Lab
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| Street address |
35 Shinanomachi
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| City |
Shinjuku |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
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| Platform ID |
GPL29836 |
| Series (1) |
| GSE168701 |
Establishment of an induced pluripotent stem cell line from a female domestic ferret (Mustela putorius furo) with an X chromosome instability |
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| Relations |
| BioSample |
SAMN18256841 |
| SRA |
SRX10312954 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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