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Sample GSM5171798 Query DataSets for GSM5171798
Status Public on Sep 21, 2023
Title E0771-EnnA1_S3
Sample type SRA
 
Source name E0771_EnnA
Organism Mus musculus
Characteristics strain background: C57BL/6
cell line: E0771
cell type: Murine breast cancer cells
treatment: EnnA
Treatment protocol Cells were treated with EnnA (7.4 µM) or 17-AAG (0.5 µM) for 24 hrs.
Growth protocol E0771 murine cells were allowed to grow in 6-well tissue culture plates for 48 hrs supplemented with complete RPMI-1640 media (Corning, # 10-040-CV) and 10% FBS.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Purelink RNA Mini kit with On-column PureLink Dnase Treatment
mRNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Illumina BCL2.0 fastq software used for basecalling.
Sequenced reads were mapped to mm9 whole genome using STAR aligner
Normalized FPKM counts were calculated using Cufflinks and Cuffdiff tools for outputting differntially expressed genes with log2 fold changes and q values.
Genome_build: mm9
Supplementary_files_format_and_content: excel file, log2 fold change
 
Submission date Mar 14, 2021
Last update date Sep 21, 2023
Contact name Ahmed Chadli
E-mail(s) achadli@augusta.edu
Phone 7067214661
Organization name Augusta University
Department Cancer Center
Street address 1410 Laney Walker BLVD
City Augusta
State/province GA
ZIP/Postal code 30912
Country USA
 
Platform ID GPL19057
Series (1)
GSE168875 A novel breast cancer therapeutic strategy through Hsp90 inhibition and activation of the immune system
Relations
BioSample SAMN18307926
SRA SRX10338992

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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