|
| Status |
Public on Sep 21, 2023 |
| Title |
E0771-EnnA2_S5 |
| Sample type |
SRA |
| |
|
| Source name |
E0771_EnnA
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
cell line: E0771
cell type: Murine breast cancer cells
treatment: EnnA
|
| Treatment protocol |
Cells were treated with EnnA (7.4 µM) or 17-AAG (0.5 µM) for 24 hrs.
|
| Growth protocol |
E0771 murine cells were allowed to grow in 6-well tissue culture plates for 48 hrs supplemented with complete RPMI-1640 media (Corning, # 10-040-CV) and 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Purelink RNA Mini kit with On-column PureLink Dnase Treatment
mRNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina BCL2.0 fastq software used for basecalling.
Sequenced reads were mapped to mm9 whole genome using STAR aligner
Normalized FPKM counts were calculated using Cufflinks and Cuffdiff tools for outputting differntially expressed genes with log2 fold changes and q values.
Genome_build: mm9
Supplementary_files_format_and_content: excel file, log2 fold change
|
| |
|
| Submission date |
Mar 14, 2021 |
| Last update date |
Sep 21, 2023 |
| Contact name |
Ahmed Chadli |
| E-mail(s) |
achadli@augusta.edu
|
| Phone |
7067214661
|
| Organization name |
Augusta University
|
| Department |
Cancer Center
|
| Street address |
1410 Laney Walker BLVD
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE168875 |
A novel breast cancer therapeutic strategy through Hsp90 inhibition and activation of the immune system |
|
| Relations |
| BioSample |
SAMN18307925 |
| SRA |
SRX10338993 |
