|
| Status |
Public on Sep 30, 2010 |
| Title |
3T3-L1_t1_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
3T3-L1 pre-adipocyte, day -2, replicate 2
|
| Organism |
Mus musculus |
| Characteristics |
cell type: 3T3-L1 pre-adipocyte
|
| Growth protocol |
3T3-L1 pre-adipocytes were maintained in DMEM supplemented with 10% calf serum. Two days after confluence, the cells were induced with DMEM supplemented with 10% FBS, 1uM dexamethasone, 1.7uM insulin, and 0.5mM 3-isobutyl-1-methylxanthine (IBMX). 48h later, the medium was replaced with DMEM supplemented with 10% FBS and the cells were fed every two days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using TRIzol (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
As recommended by Affymetrix from 1 ug total RNA/sample
|
| |
|
| Hybridization protocol |
As recommended by Affymetrix
|
| Scan protocol |
As recommended by Affymetrix
|
| Description |
no additional information
|
| Data processing |
The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
|
| |
|
| Submission date |
Mar 09, 2010 |
| Last update date |
Sep 07, 2010 |
| Contact name |
Tarjei S Mikkelsen |
| Organization name |
Broad Institute
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE20696 |
Expression profiling of 3T3-L1 adipogenesis |
| GSE20752 |
Comparative Epigenomic Analysis of Murine and Human Adipogenesis |
|
