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Sample GSM519582 Query DataSets for GSM519582
Status Public on Sep 30, 2010
Title 3T3-L1_t2_rep2
Sample type RNA
 
Source name 3T3-L1 pre-adipocyte, day 0, replicate 2
Organism Mus musculus
Characteristics cell type: 3T3-L1 pre-adipocyte
Growth protocol 3T3-L1 pre-adipocytes were maintained in DMEM supplemented with 10% calf serum. Two days after confluence, the cells were induced with DMEM supplemented with 10% FBS, 1uM dexamethasone, 1.7uM insulin, and 0.5mM 3-isobutyl-1-methylxanthine (IBMX). 48h later, the medium was replaced with DMEM supplemented with 10% FBS and the cells were fed every two days.
Extracted molecule total RNA
Extraction protocol Total RNA were extracted using TRIzol (Invitrogen)
Label biotin
Label protocol As recommended by Affymetrix from 1 ug total RNA/sample
 
Hybridization protocol As recommended by Affymetrix
Scan protocol As recommended by Affymetrix
Description no additional information
Data processing The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
 
Submission date Mar 09, 2010
Last update date Sep 07, 2010
Contact name Tarjei S Mikkelsen
Organization name Broad Institute
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL1261
Series (2)
GSE20696 Expression profiling of 3T3-L1 adipogenesis
GSE20752 Comparative Epigenomic Analysis of Murine and Human Adipogenesis

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
1415670_at 660.6573617
1415671_at 2391.984387
1415672_at 1985.84998
1415673_at 189.9094927
1415674_a_at 688.4932123
1415675_at 553.1298507
1415676_a_at 936.0000942
1415677_at 220.715751
1415678_at 744.4106948
1415679_at 2129.996381
1415680_at 830.1104161
1415681_at 826.6044824
1415682_at 136.2184553
1415683_at 1153.854482
1415684_at 194.3156355
1415685_at 241.7948764
1415686_at 630.5669769
1415687_a_at 5635.479482
1415688_at 1150.706998
1415689_s_at 407.299496

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM519582.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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