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Sample GSM519591 Query DataSets for GSM519591
Status Public on Sep 30, 2010
Title hASC_t2_rep1
Sample type RNA
 
Source name hASC pre-adipocyte, day 0
Organism Homo sapiens
Characteristics gender: female
cell type: adipose stromal cells
Growth protocol hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
Extracted molecule total RNA
Extraction protocol Total RNA were extracted using TRIzol (Invitrogen)
Label biotin
Label protocol As recommended by Affymetrix from 1 ug total RNA/sample
 
Hybridization protocol As recommended by Affymetrix
Scan protocol As recommended by Affymetrix
Description no additional information
Data processing The data were extracted and RMA normalized using GenePattern (http://genepattern.broadinstitute.org)
 
Submission date Mar 09, 2010
Last update date Sep 07, 2010
Contact name Tarjei S Mikkelsen
Organization name Broad Institute
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL570
Series (2)
GSE20697 Expression profiling of human adipose stromal cell (hASC) adipogenesis
GSE20752 Comparative Epigenomic Analysis of Murine and Human Adipogenesis

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
1007_s_at 162.9784486
1053_at 97.63085592
117_at 11.42407452
121_at 123.3954876
1255_g_at 11.39294907
1294_at 75.49387234
1316_at 100.5668131
1320_at 17.18851434
1405_i_at 8.235822846
1431_at 10.93709157
1438_at 47.21230522
1487_at 61.32977583
1494_f_at 24.29751499
1552256_a_at 56.12486058
1552257_a_at 253.9732201
1552258_at 15.39443644
1552261_at 13.79139724
1552263_at 46.48113938
1552264_a_at 313.0137747
1552266_at 13.12660794

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM519591.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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