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Sample GSM519611 Query DataSets for GSM519611
Status Public on Sep 30, 2010
Title hASC_t1_rep2
Sample type RNA
 
Source name hASC pre-adipocyte, day -2
Organism Homo sapiens
Characteristics gender: female
cell type: adipose stromal cells
Treatment protocol N/A
Growth protocol hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
Extracted molecule total RNA
Extraction protocol Total RNA were extracted using TRIzol (Invitrogen) and subjected to miRNA-DASL
Label Cy3
Label protocol As recommended by Illumina
 
Hybridization protocol As recommended by Illumina
Scan protocol As recommended by Illumina
Description no additional information
Data processing The data were extracted, background subtracted and quantile normalzied using GenomeStudio 2009 (Illumina)
 
Submission date Mar 09, 2010
Last update date Sep 07, 2010
Contact name Tarjei S Mikkelsen
Organization name Broad Institute
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL8178
Series (2)
GSE20699 MicroRNA expression profiling of human adipose stromal cell (hASC) adipogenesis
GSE20752 Comparative Epigenomic Analysis of Murine and Human Adipogenesis

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_3167304 -33.13844 0.8674872
ILMN_3168038 38.12471 0.1348094
ILMN_3167890 26962.6 3.68E-38
ILMN_3167526 -21.4016 0.7731333
ILMN_3167209 -21.87529 0.7775521
ILMN_3167213 34.80893 0.1584822
ILMN_3167057 1075.335 3.68E-38
ILMN_3168313 -28.71739 0.8357048
ILMN_3166972 -15.29634 0.7119666
ILMN_3168149 25.5984 0.2376205
ILMN_3168527 -29.08581 0.8385282
ILMN_3167562 -6.612127 0.6136274
ILMN_3168099 206.4931 1.11E-10
ILMN_3168017 46.54577 0.08595362
ILMN_3167951 516.0195 3.68E-38
ILMN_3167172 69.28261 0.01904263
ILMN_3167994 -8.243707 0.6329134
ILMN_3166997 73.33524 0.01389988
ILMN_3166980 43.01945 0.1044997
ILMN_3168362 64.17735 0.02774619

Total number of rows: 734

Table truncated, full table size 22 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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