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Sample GSM519612 Query DataSets for GSM519612
Status Public on Sep 30, 2010
Title hASC_t2_rep1
Sample type RNA
Source name hASC pre-adipocyte, day 0
Organism Homo sapiens
Characteristics gender: female
cell type: adipose stromal cells
Treatment protocol N/A
Growth protocol hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
Extracted molecule total RNA
Extraction protocol Total RNA were extracted using TRIzol (Invitrogen) and subjected to miRNA-DASL
Label Cy3
Label protocol As recommended by Illumina
Hybridization protocol As recommended by Illumina
Scan protocol As recommended by Illumina
Description no additional information
Data processing The data were extracted, background subtracted and quantile normalzied using GenomeStudio 2009 (Illumina)
Submission date Mar 09, 2010
Last update date Sep 07, 2010
Contact name Tarjei S Mikkelsen
Organization name Broad Institute
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platform ID GPL8178
Series (2)
GSE20699 MicroRNA expression profiling of human adipose stromal cell (hASC) adipogenesis
GSE20752 Comparative Epigenomic Analysis of Murine and Human Adipogenesis

Data table header descriptions
VALUE quantile normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_3167304 -32.34897 0.8592018
ILMN_3168038 29.75629 0.1249335
ILMN_3167890 22507.91 3.68E-38
ILMN_3167526 -20.24371 0.7397529
ILMN_3167209 -24.98055 0.7917393
ILMN_3167213 4.861557 0.398273
ILMN_3167057 1441.23 3.68E-38
ILMN_3168313 -27.87529 0.8202429
ILMN_3166972 -11.87529 0.6339934
ILMN_3168149 28.28261 0.1361418
ILMN_3168527 -23.4016 0.7751281
ILMN_3167562 1.861557 0.4402937
ILMN_3168099 262.3879 1.13E-21
ILMN_3168017 53.5984 0.02244016
ILMN_3167951 221.4405 5.11E-16
ILMN_3167172 217.23 1.73E-15
ILMN_3167994 70.33524 0.004579794
ILMN_3166997 72.44051 0.00366417
ILMN_3166980 -1.980548 0.4950425
ILMN_3168362 50.12471 0.02997304

Total number of rows: 734

Table truncated, full table size 22 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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