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Sample GSM519614 Query DataSets for GSM519614
Status Public on Sep 30, 2010
Title hASC_t3_rep1
Sample type RNA
 
Source name hASC pre-adipocyte, day 1
Organism Homo sapiens
Characteristics gender: female
cell type: adipose stromal cells
Treatment protocol N/A
Growth protocol hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
Extracted molecule total RNA
Extraction protocol Total RNA were extracted using TRIzol (Invitrogen) and subjected to miRNA-DASL
Label Cy3
Label protocol As recommended by Illumina
 
Hybridization protocol As recommended by Illumina
Scan protocol As recommended by Illumina
Description no additional information
Data processing The data were extracted, background subtracted and quantile normalzied using GenomeStudio 2009 (Illumina)
 
Submission date Mar 09, 2010
Last update date Sep 07, 2010
Contact name Tarjei S Mikkelsen
Organization name Broad Institute
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL8178
Series (2)
GSE20699 MicroRNA expression profiling of human adipose stromal cell (hASC) adipogenesis
GSE20752 Comparative Epigenomic Analysis of Murine and Human Adipogenesis

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_3167304 -36.13844 0.8880138
ILMN_3168038 28.54577 0.1123052
ILMN_3167890 21561.49 3.68E-38
ILMN_3167526 7.598399 0.3345804
ILMN_3167209 -0.03317998 0.4441098
ILMN_3167213 14.86156 0.2419011
ILMN_3167057 1528.493 3.68E-38
ILMN_3168313 -26.82265 0.8067575
ILMN_3166972 -11.03318 0.6074765
ILMN_3168149 32.01945 0.08933248
ILMN_3168527 -33.13844 0.8650545
ILMN_3167562 -24.03318 0.7767312
ILMN_3168099 282.3352 3.03E-27
ILMN_3168017 68.38787 0.003350487
ILMN_3167951 206.4931 1.40E-15
ILMN_3167172 -22.71739 0.7616938
ILMN_3167994 -3.612127 0.4975725
ILMN_3166997 3.493136 0.3924066
ILMN_3166980 -8.454232 0.5697936
ILMN_3168362 49.33524 0.02299482

Total number of rows: 734

Table truncated, full table size 22 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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