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Sample GSM519619 Query DataSets for GSM519619
Status Public on Sep 30, 2010
Title hASC_t5_rep2
Sample type RNA
Source name hASC pre-adipocyte, day 3
Organism Homo sapiens
Characteristics gender: female
cell type: adipose stromal cells
Treatment protocol N/A
Growth protocol hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
Extracted molecule total RNA
Extraction protocol Total RNA were extracted using TRIzol (Invitrogen) and subjected to miRNA-DASL
Label Cy3
Label protocol As recommended by Illumina
Hybridization protocol As recommended by Illumina
Scan protocol As recommended by Illumina
Description no additional information
Data processing The data were extracted, background subtracted and quantile normalzied using GenomeStudio 2009 (Illumina)
Submission date Mar 09, 2010
Last update date Sep 07, 2010
Contact name Tarjei S Mikkelsen
Organization name Broad Institute
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platform ID GPL8178
Series (2)
GSE20699 MicroRNA expression profiling of human adipose stromal cell (hASC) adipogenesis
GSE20752 Comparative Epigenomic Analysis of Murine and Human Adipogenesis

Data table header descriptions
VALUE quantile normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_3167304 -37.13844 0.9597866
ILMN_3168038 -0.8226537 0.1570994
ILMN_3167890 26962.6 3.68E-38
ILMN_3167526 -3.243706 0.205309
ILMN_3167209 -22.29634 0.7331588
ILMN_3167213 27.38787 0.0008268
ILMN_3167057 2581.23 3.68E-38
ILMN_3168313 -26.61213 0.8288831
ILMN_3166972 -7.243706 0.301743
ILMN_3168149 30.4405 0.000365317
ILMN_3168527 -30.34897 0.8912515
ILMN_3167562 -19.92792 0.671024
ILMN_3168099 227.7037 3.68E-38
ILMN_3168017 61.54577 4.82E-09
ILMN_3167951 98.65103 6.13E-18
ILMN_3167172 -17.03318 0.5882975
ILMN_3167994 1.861557 0.1131287
ILMN_3166997 113.8089 1.51E-22
ILMN_3166980 5.019452 0.07358613
ILMN_3168362 106.4405 3.09E-20

Total number of rows: 734

Table truncated, full table size 22 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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