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Sample GSM519621 Query DataSets for GSM519621
Status Public on Sep 30, 2010
Title hASC_t6_rep2
Sample type RNA
Source name hASC pre-adipocyte, day 5
Organism Homo sapiens
Characteristics gender: female
cell type: adipose stromal cells
Treatment protocol N/A
Growth protocol hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
Extracted molecule total RNA
Extraction protocol Total RNA were extracted using TRIzol (Invitrogen) and subjected to miRNA-DASL
Label Cy3
Label protocol As recommended by Illumina
Hybridization protocol As recommended by Illumina
Scan protocol As recommended by Illumina
Description no additional information
Data processing The data were extracted, background subtracted and quantile normalzied using GenomeStudio 2009 (Illumina)
Submission date Mar 09, 2010
Last update date Sep 07, 2010
Contact name Tarjei S Mikkelsen
Organization name Broad Institute
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platform ID GPL8178
Series (2)
GSE20699 MicroRNA expression profiling of human adipose stromal cell (hASC) adipogenesis
GSE20752 Comparative Epigenomic Analysis of Murine and Human Adipogenesis

Data table header descriptions
VALUE quantile normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_3167304 -29.45423 0.8671803
ILMN_3168038 -20.71739 0.789735
ILMN_3167890 26962.6 3.68E-38
ILMN_3167526 -23.82265 0.819865
ILMN_3167209 10.54577 0.3838449
ILMN_3167213 17.5984 0.2933069
ILMN_3167057 2178.44 3.68E-38
ILMN_3168313 -26.82265 0.8462526
ILMN_3166972 -9.138443 0.6545968
ILMN_3168149 10.33524 0.3866793
ILMN_3168527 -28.71739 0.8615288
ILMN_3167562 -13.92792 0.7144451
ILMN_3168099 162.3352 8.50E-09
ILMN_3168017 37.44051 0.1070318
ILMN_3167951 144.8616 2.52E-07
ILMN_3167172 93.12472 0.000679301
ILMN_3167994 4.861557 0.4620642
ILMN_3166997 7.072083 0.4312958
ILMN_3166980 83.65103 0.002054205
ILMN_3168362 61.54577 0.01825002

Total number of rows: 734

Table truncated, full table size 22 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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