hASCs were grown in MesenPRO RS medium (Invitrogen) plus 4ng/ml FGF-2 on plates coated with 10ug/cm2 collagen I (Invitrogen). Two days after confluence, the cells were placed in induction medium (DMEM/F12 supplemented with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 0.5mM IBMX, 5uM rosiglitazone, 40ng/ml BMP4, 17uM pantothenic acid, and 33uM biotin). 72h later, the medium was replaced with maintenance medium (DMEM/F12 with 10% FBS plus 1uM dexamethasone, 1.7uM insulin, 17uM pantothenic acid, and 33uM biotin) and media was replaced every two days thereafter.
Extracted molecule
total RNA
Extraction protocol
Total RNA were extracted using TRIzol (Invitrogen) and subjected to miRNA-DASL
Label
Cy3
Label protocol
As recommended by Illumina
Hybridization protocol
As recommended by Illumina
Scan protocol
As recommended by Illumina
Description
no additional information
Data processing
The data were extracted, background subtracted and quantile normalzied using GenomeStudio 2009 (Illumina)
