|
| Status |
Public on Jan 12, 2022 |
| Title |
4705_P |
| Sample type |
genomic |
| |
|
| Source name |
prefrontal cortex, neuron, TG mouse
|
| Organism |
Mus musculus |
| Characteristics |
cell type: prefrontal cortex, NeuN+, mC+
profile: Control
fractions: neuronal nuclei
|
| Treatment protocol |
Fresh brain samples were separated into neuronal and noneuronal nuclei by NeuN-based cell sorting. Enrichment of methylated DNA was performed using MethylCollector (Active Motif).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Standard phenol–chloroform extraction protocols.
|
| Label |
biotin
|
| Label protocol |
According to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
According to the standard Affymetrix protocol.
|
| Scan protocol |
According to the standard Affymetrix protocol.
|
| Data processing |
The signal intensity data was analyzed with the MAT Software, using parameters: Bandwith= 300bp, Max Gap= 300, Min Probe= 10.Pvalue = 1e-3.
|
| |
|
| Submission date |
Mar 30, 2021 |
| Last update date |
Jan 12, 2022 |
| Contact name |
Junko Ueda |
| E-mail(s) |
junko.hayashi.aa@riken.jp
|
| Organization name |
RIKEN
|
| Department |
Center for Brain Science
|
| Lab |
Laboratory for Molecular Dynamics of Mental Disorders
|
| Street address |
2-1 Hirosawa
|
| City |
Wako |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
|
| Platform ID |
GPL5811 |
| Series (1) |
| GSE171120 |
Promoter-wide profiling of DNA methylation in prefrontal cortex of Camk2-mutPolg mouse. |
|
