|
| Status |
Public on Apr 01, 2024 |
| Title |
H2O2-treated rep1 |
| Sample type |
RNA |
| |
|
| Source name |
S. oligofermentans, anaerobically grown, treated with H2O2, repeat 1
|
| Organism |
Streptococcus cristatus |
| Characteristics |
strain: AS 1.3089
treatment: H2O2-treated
|
| Treatment protocol |
One aliquot of S. oligofermentans was treated with 0.5 mM H2O2, leaving another aliquot untreated.
|
| Growth protocol |
S. oligofermentans was anaerobically grown under 100% N2 in Brain Heart Infusion broth
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent (Invitrogen) following the manufacturer's recommendations. Qualified total RNA was further purified using RNeasy micro kit (QIAGEN, GmBH, Germany) and RNase-Free DNase Set (QIAGEN, GmBH, Germany)
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared using Low Input Quick Amp Labeling Kit, One-Color (Agilent technologies), and purified using RNeasy mini kit (QIAGEN).
|
| |
|
| Hybridization protocol |
Each microarray chip slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies) according to the manufacturer’s instructions. After 17 h hybridization, slides were washed with Gene Expression Wash Buffer Kit,
|
| Scan protocol |
Microarray chips were scanned by Agilent Microarray Scanner with default settings.
|
| Description |
Gene expression profiles in H2O2 treated anaerobically grown S. oligofermentans
|
| Data processing |
Data were extracted with Feature Extraction software 10.7 (Agilent technologies), and normalized using Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies).
|
| |
|
| Submission date |
Apr 07, 2021 |
| Last update date |
Apr 01, 2024 |
| Contact name |
Huichun Tong |
| E-mail(s) |
tonghuichun00@sina.com
|
| Phone |
+861064807567
|
| Organization name |
Institute of Microbiology, Chinese Academy of Sciences
|
| Street address |
NO. 3, Yihao Yuan, Beichen West Road, Chaoyang District, Beijing
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL29985 |
| Series (1) |
| GSE171631 |
Evaluating the H2O2-induced tRNA expressions change in Streptococcus oligofermentans |
|
