|
| Status |
Public on Apr 01, 2010 |
| Title |
mp53 + IR, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
mp53 + IR
|
| Organism |
Mus musculus |
| Characteristics |
cell type: R26-mp53 (fully recombined)
competitive status: no competition
treatment: irradiated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 200 sorted cells by standard Trizol protocol (Invitrogen) with addition of glycogen, then amplified using RiboAmpHS kit (from Arcturus)
|
| Label |
biotin
|
| Label protocol |
biotin label was incorporated by in vitro transcription during the second round of RNA amplification
|
| |
|
| Hybridization protocol |
1.5 ug of cRNA were hybridized to Illumina Mouse-6 Expression BeadChip v1.1 by standard Illumina protocol
|
| Scan protocol |
standard Illumina protocol
|
| Description |
mp53 + IR, biological replicate 1
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 16, 2010 |
| Last update date |
Mar 16, 2010 |
| Contact name |
Tanya Bondar |
| Organization name |
Yale University
|
| Street address |
300 Cedar str TAC S660
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE20912 |
p53-mediated hematopoietic stem and progenitor cell competition |
|
