|
| Status |
Public on Apr 03, 2024 |
| Title |
A549, 10 ng/ml IL4 + Compound A_1 |
| Sample type |
SRA |
| |
|
| Source name |
A549_IL4+Compound treated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: Lung epithelial Adenocarcinoma (Alveolar Type II like)
treatment: IL4+Compound treated
|
| Treatment protocol |
A549 cells were seeded in 6 well plates @ 4 x 10^5 cells/well in 2 ml media; treated with +/- 10 ng/ml IL4 and +/- 1 uM Smartpool ALOX15 siRNA for 48 hrs before mRNA extraction.
|
| Growth protocol |
A549 cells were grown in F12 (Ham's) media containing 10% HI FBS supplemented with 1x non-essential amino acids (Invitrogen) and 1x Pen/Strep glutamine (Invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy mini prep kit
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
CMPD_1
SE6999_SA81563_S11_L008
|
| Data processing |
The reads were first mapped to the latest UCSC transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include raw count values for each Sample
|
| |
|
| Submission date |
Apr 08, 2021 |
| Last update date |
Apr 03, 2024 |
| Contact name |
Jonathan Phillips |
| Organization name |
Amgen
|
| Street address |
1 Amgen center drive
|
| City |
Thousand Oaks |
| State/province |
CA |
| ZIP/Postal code |
91320 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE171750 |
A549 cell Next Gen sequencing (RNA-seq) for differential gene expression of untreated versus IL4 or IL4+ALOX15 siRNA treated cells |
|
| Relations |
| BioSample |
SAMN18678378 |
| SRA |
SRX10560399 |
