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Sample GSM5233384 Query DataSets for GSM5233384
Status Public on Apr 03, 2024
Title A549, 10 ng/ml IL4 + Control Smartpool siRNA_1
Sample type SRA
 
Source name A549_IL4+non-targeting negative control siRNA
Organism Homo sapiens
Characteristics cell line: A549
cell type: Lung epithelial Adenocarcinoma (Alveolar Type II like)
treatment: IL4+non-targeting negative control siRNA
Treatment protocol A549 cells were seeded in 6 well plates @ 4 x 10^5 cells/well in 2 ml media; treated with +/- 10 ng/ml IL4 and +/- 1 uM Smartpool ALOX15 siRNA for 48 hrs before mRNA extraction.
Growth protocol A549 cells were grown in F12 (Ham's) media containing 10% HI FBS supplemented with 1x non-essential amino acids (Invitrogen) and 1x Pen/Strep glutamine (Invitrogen)
Extracted molecule total RNA
Extraction protocol Qiagen RNAeasy mini prep kit
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description Control_siRNA_1
SE6999_SA81564_S12_L008
Data processing The reads were first mapped to the latest UCSC transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include raw count values for each Sample
 
Submission date Apr 08, 2021
Last update date Apr 03, 2024
Contact name Jonathan Phillips
Organization name Amgen
Street address 1 Amgen center drive
City Thousand Oaks
State/province CA
ZIP/Postal code 91320
Country USA
 
Platform ID GPL20301
Series (1)
GSE171750 A549 cell Next Gen sequencing (RNA-seq) for differential gene expression of untreated versus IL4 or IL4+ALOX15 siRNA treated cells
Relations
BioSample SAMN18678316
SRA SRX10560402

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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