|
Status |
Public on Aug 17, 2010 |
Title |
Mouse lung-embryo day 14-rep1 |
Sample type |
RNA |
|
|
Source name |
embryo lung
|
Organism |
Mus musculus |
Characteristics |
strain: ICR gender: female development stage: embryo day 14
|
Treatment protocol |
Lungs were isolated by manual dissection with the aid of a dissecting microscope where necessary. Lungs were washed twice with PBS before total RNA extraction
|
Growth protocol |
ICR female mice were bred for timed pregnancies. The day of plug observation was considered day 0 of gestation.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Labeling was performed according to Affymetrix Gene Chip technical manual
|
|
|
Hybridization protocol |
The targets for Affymetrix DNA microarray analysis were prepared according to the manufacturer’s instructions. Biotin-labeled cRNA, produced by in vitro transcription, was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays at 45°C for 16 hr and then washed and stained using the GeneChip Fluidics.
|
Scan protocol |
The arrays were scanned by a GeneArray Scanner and patterns of hybridization detected as light emitted from the fluorescent reporter groups incorporated into the target and hybridized to oligonucleotide probes.
|
Description |
Gene expression data from different lung development stages
|
Data processing |
The data were analyzed with Partek Genomics Suite 6.4 using Affymetrix default analysis settings and GC-RMA as normalization method
|
|
|
Submission date |
Mar 18, 2010 |
Last update date |
Aug 17, 2010 |
Contact name |
Jie Dong |
E-mail(s) |
dong.jie@mayo.edu
|
Organization name |
Mayo Clinic
|
Department |
Thoracic Surgery
|
Street address |
200 1st SW
|
City |
Rochester |
State/province |
MN |
ZIP/Postal code |
55901 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE20954 |
mRNA expression profile in mouse lung development |
GSE21053 |
mRNA and microRNA expression profile in mouse lung development |
|