GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM525485 Query DataSets for GSM525485
Status Public on Dec 31, 2011
Title (745)Morphology:Brachial artery of pig#9481
Sample type RNA
Source name Brachial artery of pig#9481
Organism Sus scrofa
Characteristics artery: Brachial artery
pig id: 9481
cell type: blood, whole
age: 1 week
Growth protocol Pigs were on a normal diet
Extracted molecule total RNA
Extraction protocol RNA was isolated using Macherey Nagel Nucleo Spin II for the piglets and CsCl ultracentrifugation followed by MN Nucleospin II for the one and two year olds.
Label Biotin
Label protocol Biotinylated cRNA were prepared by the GeneChip One-Cycle target labeling kit according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol Following fragmentation, 6.5 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 (affymetrix).
Description Brachial artery of pig#9481
Data processing Default GCOS 1.4 raw probe intensities were analyzed using the mas5 module of the affy 1.0 package for R version 2.3.1. The probesets that were present according to MAS5calls and normalized, background corrected, and summarized using the RMA function in Bionconductor.
Submission date Mar 24, 2010
Last update date Dec 31, 2011
Contact name Martin Bahls
Organization name Purdue University
Street address 800 W Stadium Ave
City West Lafayette
ZIP/Postal code 47906
Country USA
Platform ID GPL3533
Series (1)
GSE21043 Gene expression differences between the brachial and femoral artery of the Rapacz HC swine

Data table header descriptions
VALUE normalized data as described in the data processing

Data table
AFFX-BioB-3_at 10.06296998
AFFX-BioB-5_at 10.10785163
AFFX-BioB-M_at 10.24344294
AFFX-BioC-3_at 10.28058887
AFFX-BioC-5_at 10.55022005
AFFX-BioDn-3_at 12.88507266
AFFX-BioDn-5_at 11.26178675
AFFX-CreX-3_at 13.9746077
AFFX-CreX-5_at 13.74169224
AFFX-DapX-3_at 11.49194896
AFFX-DapX-5_at 10.58029628
AFFX-DapX-M_at 11.73180828
AFFX-LysX-3_at 9.145350884
AFFX-LysX-5_at 7.344816494
AFFX-LysX-M_at 7.319266101
AFFX-PheX-3_at 8.465830463
AFFX-PheX-5_at 8.20262217
AFFX-PheX-M_at 8.526670076
AFFX-r2-Bs-dap-3_at 11.82610588
AFFX-r2-Bs-dap-5_at 10.73420036

Total number of rows: 19459

Table truncated, full table size 563 Kbytes.

Supplementary file Size Download File type/resource
GSM525485.CEL.gz 2.2 Mb (ftp)(http) CEL
GSM525485.CHP.gz 127.4 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap