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Sample GSM5261214 Query DataSets for GSM5261214
Status Public on Apr 30, 2023
Title MPP_b_FL
Sample type RNA
 
Source name Fetal liver
Organism Mus musculus
Characteristics cell type: Fetal liver MPP cells
developmental stage: E13.5
strain: C57Bl/6J
Growth protocol Fetal liver cells were isolated from e13.5 embryos. Bone marrow was isolated from mice 3 to 12 months old.
Extracted molecule total RNA
Extraction protocol RNeasy Micro Kit (Qiagen No. 74004).
Label biotin
Label protocol cRNA was prepared according to the standard Affymetrix protocol from 2 ng total RNA (GeneChIP Pico Reagent Kit, Affymetrix). 10 cycles were used for Pre IVT-PCR.
 
Hybridization protocol Cartridge Array Hybridization was performed according to standard Affymetrix protocols (Affymetrix Pico Kit User Guide (P/N 703262) for Array Plate Hybridization).
Scan protocol Arrays were scanned using the Affymetrix GCS3000 Scanner (Software GCOS 1.2).
Description Heckl3031
Gene expression data from BM transplanted mice transduced with BRSST sgRNA +sgEzh2
Data processing The data were analyzed using R 3.5.01 and Bioconductor2. Raw files (.cel) were read in using the function “read.celfiles” from the R package “oligo”. Quality control was performed by inspection of the RLE (Relative Log Expression ) plots and NUSE (normalized unscaled standard error) plots after fitting a Probe Level Model to the raw intensity data by using the function “fitProbeLevelModel” from the package “oligo”. The Raw data was background corrected, quantile-normalized, and log2-transformed using the function “rma” from the ”oligo” package.
 
Submission date Apr 22, 2021
Last update date Apr 30, 2023
Contact name Dorit Borchert
E-mail(s) Borchert.Dorit@mh-hannover.de
Organization name Hannover Medical School
Department Pediatric Hematology and Oncology
Street address Carl-Neuberg-Str. 1
City Hannover
ZIP/Postal code 30625
Country Germany
 
Platform ID GPL23038
Series (2)
GSE173158 Expression data of freshly isolated and sorted HSC populations from murine fetal liver or adult bone marrow [array]
GSE173497 Oncofetal gene reactivation drives EZH2-mutant myeloid leukemia pathogenesis

Data table header descriptions
ID_REF
VALUE Normalized value

Data table
ID_REF VALUE
TC0100000014.mm.2 8.180714459
TC0100000018.mm.2 5.959825344
TC0100000021.mm.2 8.729045188
TC0100000022.mm.2 5.397518273
TC0100000023.mm.2 4.429347988
TC0100000027.mm.2 8.259710171
TC0100000038.mm.2 9.849218541
TC0100000039.mm.2 6.93220083
TC0100000041.mm.2 6.642503268
TC0100000044.mm.2 9.275249983
TC0100000045.mm.2 5.914550546
TC0100000048.mm.2 7.765713468
TC0100000055.mm.2 6.76943964
TC0100000056.mm.2 6.211993165
TC0100000064.mm.2 6.029933286
TC0100000071.mm.2 5.11631513
TC0100000082.mm.2 6.268552369
TC0100000092.mm.2 8.737203355
TC0100000097.mm.2 7.587805868
TC0100000101.mm.2 9.130472903

Total number of rows: 22203

Table truncated, full table size 649 Kbytes.




Supplementary file Size Download File type/resource
GSM5261214_Heckl3031_Clariom_S_Mouse_.CEL.gz 1.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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