|
Status |
Public on Aug 17, 2010 |
Title |
E18-2cardA/B |
Sample type |
RNA |
|
|
Source name |
embryo lung
|
Organism |
Mus musculus |
Characteristics |
strain: ICR gender: female development stage: embryo day 18
|
Treatment protocol |
Lungs were isolated by manual dissection with the aid of a dissecting microscope where necessary. Lungs were washed twice with PBS before total RNA extraction
|
Growth protocol |
ICR female mice were bred for timed pregnancies. The day of plug observation was considered day 0 of gestation.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using mir VanaTM miRNA Isolation Kit (Ambion) according to the manufacturer's instructions.
|
Label |
SYBR
|
Label protocol |
none
|
|
|
Hybridization protocol |
not applicable
|
Scan protocol |
Raw miRNA array data was analyzed by using RQ manager software on the ABI system
|
Description |
micro expression data from different lung development stages reverse_transcription_protocol: MiRNA was reverse transcibed to cDNA using the Megaplex TM RT Rodent Primers Pool and the TaqMan MicroRNA Reverse Transcription Kit real-time_PCR: Quantitative 384 well TaqMan Low Density Array real-time PCR was run on the ABI PRISM 7900 System using the TaqMan Universal PCR Master Mix
|
Data processing |
Normalized CT (ΔCT) was calculated by comparing each miRNA value to that of small nuclear U6 RNA. The U6 RNA is a common internal control for each microRNA array card.
|
|
|
Submission date |
Mar 24, 2010 |
Last update date |
Aug 17, 2010 |
Contact name |
Jie Dong |
E-mail(s) |
dong.jie@mayo.edu
|
Organization name |
Mayo Clinic
|
Department |
Thoracic Surgery
|
Street address |
200 1st SW
|
City |
Rochester |
State/province |
MN |
ZIP/Postal code |
55901 |
Country |
USA |
|
|
Platform ID |
GPL10253 |
Series (2) |
GSE21052 |
microRNA expression profile in mouse lung development |
GSE21053 |
mRNA and microRNA expression profile in mouse lung development |
|