|
| Status |
Public on Apr 01, 2022 |
| Title |
Case in remission RC15 |
| Sample type |
RNA |
| |
|
| Source name |
Case in remission
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Frozen lymph node biopsy
disease state: Diffuse large B-cell lymphoma (DLBCL)
response to r-chop: remission
|
| Treatment protocol |
Diffuse large B-cell lymphoma (DLBCL) patient samples from frozen lymph node biopsies.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
RNA samples were processed to Affymetrix GeneChip HT HG-U219 arrays.
|
| Scan protocol |
Scanning was processed in the Gene Titan instrument.
|
| Description |
Gene expression data from DLBCL cases in remission
|
| Data processing |
Determination of the signal value for each probe set of the array were obtained with GeneChip® Command Console® Software (AGCC) (Affymetrix).
Raw data were normalized using the Robust Multichip Analysis (RMA) algorithm implemented in the Expression Console Software v1.1 (Affymetrix).
|
| |
|
| Submission date |
Apr 25, 2021 |
| Last update date |
Apr 01, 2022 |
| Contact name |
Ivan Dlouhy |
| E-mail(s) |
burguesbcn@gmail.com
|
| Organization name |
IPO Lisboa
|
| Department |
Hematology
|
| Street address |
Rua Prof Lima Basto
|
| City |
Lisboa |
| ZIP/Postal code |
1099-023 |
| Country |
Portugal |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE173263 |
Gene expression profile in DLBCL cases according to response to R-CHOP |
|
