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Sample GSM5271568 Query DataSets for GSM5271568
Status Public on Apr 21, 2024
Title Gα12KO+APAP, liver 1
Sample type SRA
 
Source name liver
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: liver
age: 10-12 weeks
genotype: G<alpha>12-/-
treatment: acetaminophen
Extracted molecule total RNA
Extraction protocol TruSeq Stranded mRNA LT Sample Prep Kit.
RNA libraries were prepared for sequencing using standard Illumina protocols and TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description 2Ga12KO_APAP
Gα12-/-
Data processing FastQC v0.11.7 used for base-calling and quality check.
Trimmomatic 0.38 performs trimming and alignment with various parameters for Illumina paired-end or single-end.
HISAT2 version 2.1.0, Bowtie2 2.3.4.1 is a tool that quickly and accurately maps high throughput sequencing data to the reference genome.
StringTie version 2.1.3b is a tool that assembles sequencing information mapped to a reference to quickly and accurately generate potential transcripts.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: Expression_Profile.mm10.gene.txt: Tab-delimited text file includes Read_Count, FPKM (normalized value), and TPM (normalized value) values for each sample.
 
Submission date Apr 29, 2021
Last update date Apr 21, 2024
Contact name Sang Geon Kim
E-mail(s) sgk@snu.ac.kr
Phone +82-2-880-7840
Organization name Seoul National University
Department College of Pharmacy
Lab Lab of Molecular Pharmacology
Street address San56-1, Sillim-dong, Kwanak-gu
City Seoul
State/province Seoul
ZIP/Postal code 151-742
Country South Korea
 
Platform ID GPL17021
Series (1)
GSE173595 Gene expression data from acetaminophen-induced toxicity in wild-type and Gα12 knockout mice
Relations
BioSample SAMN18926183
SRA SRX10715021

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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