|
| Status |
Public on May 06, 2024 |
| Title |
Sample 3_VL51-IDE-R-R1 |
| Sample type |
SRA |
| |
|
| Source name |
VL51_Resistant
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: VL51
condition: Resistant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling. Four fastq files per samples.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg38 whole genome using STAR with default paramenters. Genes' Expression have been quantified using HT-Seq count using gencode v22 as gtf.
Genome_build: hg38
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
May 06, 2021 |
| Last update date |
May 06, 2024 |
| Contact name |
Luciano Cascione |
| E-mail(s) |
luciano.cascione@ior.usi.ch
|
| Organization name |
IOR
|
| Department |
Lymphoma & Genomics Research Program
|
| Street address |
Via Chiesa 5
|
| City |
Bellinzona |
| State/province |
Ticino |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE173984 |
RNA-Seq of B-Cell Lines Clones, parental and resistant to Idelalisib or IMGN529 |
|
| Relations |
| BioSample |
SAMN19037141 |
| SRA |
SRX10808465 |
