|
| Status |
Public on Jun 14, 2010 |
| Title |
NSC_FBS_V1 |
| Sample type |
RNA |
| |
|
| Source name |
p53-null NSC, vector
|
| Organism |
Mus musculus |
| Characteristics |
cell type: primary p53-null neural stem cells
retroviral expression: vector
|
| Treatment protocol |
p53-null NSCs were infected with retrovirus expressing either control vector or PlagL2, and cultured in 1% FBS for 24 hr.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA followed by Rneasy column cleanup was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Vector-1
|
| Data processing |
Arrays were normalized using MAS5.
|
| |
|
| Submission date |
Mar 30, 2010 |
| Last update date |
Jun 14, 2010 |
| Contact name |
Haoqiang Ying |
| E-mail(s) |
haoqiang_ying@dfci.harvard.edu
|
| Phone |
617-582-7978
|
| Fax |
617-632-6069
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Lab |
Ronald DePinho
|
| Street address |
44 Binney St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE21143 |
PLAGL2 regulates Wnt signaling to impede differentiation in neural stem cells and glioma |
|
