|
Status |
Public on May 15, 2021 |
Title |
Wildtype |
Sample type |
SRA |
|
|
Source name |
Inguinal Adipose Tissue
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 treatment: Cold acclimated followed by 1wk 6C genotype/variation: Wildtype
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Adipose tissue is digested and enriched for the mature fraction. Adipocytes are then processed for nuclei isolation. 10X genomics scATAC-seq library construction protocol
|
|
|
Library strategy |
ATAC-seq |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Cell Ranger ATAC Cell Ranger produces a count matrix consisting of the counts of fragment ends within each peak region for each barcode. The matrix is then filtered to consist of only cell barcodes, and dimensional reduction is performed via LSA on the filtered peak-barcode matrix consisting of cut site counts for cell barcodes. Genome_build: mm10 Supplementary_files_format_and_content: filtered_peak_bc_matrix
|
|
|
Submission date |
May 14, 2021 |
Last update date |
May 15, 2021 |
Contact name |
Kosaku Shinoda |
E-mail(s) |
bonitoo@gmail.com
|
Phone |
7186781189
|
Organization name |
Albert Einstein College of Medicine
|
Lab |
Shinoda lab
|
Street address |
1301 Morris Park Ave
|
City |
Bronx |
State/province |
NY |
ZIP/Postal code |
10461 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE174456 |
scATAC-seq of Inguinal Adipose Tissue of UCP1-KO and WT mice. |
|
Relations |
BioSample |
SAMN19193737 |
SRA |
SRX10883754 |