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| Status |
Public on Jun 30, 2023 |
| Title |
decidualized hESCs, 12-2CD4 |
| Sample type |
SRA |
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| Source name |
hESCs, 12-2CD4
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| Organism |
Homo sapiens |
| Characteristics |
cell type: human endometrial stromal cells
cell state: induced decidualization
treatment: si-circFAM120A
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using using Trizol reagent following the manufacturer's protocol.
The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Raw reads were processed using Trimmomatic. The reads containing ploy-N and the low quality reads were removed to obtain the clean reads. The clean reads were mapped to reference genome using hisat 2.
FPKM value of each gene was calculated using cufflinks, and the read counts of each gene were obtained by htseq-count.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
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| Submission date |
May 15, 2021 |
| Last update date |
Jun 30, 2023 |
| Contact name |
Tingting Zhou |
| E-mail(s) |
tingtingzhouhelen@hotmail.com
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| Phone |
+86 15673187412
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| Organization name |
Shandong
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| Street address |
157 Jingliu Road
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| City |
Jinan |
| ZIP/Postal code |
250012 |
| Country |
China |
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| Platform ID |
GPL24676 |
| Series (1) |
| GSE174495 |
Next Generation Sequencing of si-NC and si-circFAM120A Transcriptomes |
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| Relations |
| BioSample |
SAMN19222196 |
| SRA |
SRX10897759 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM5315979_12-2CD4_FPKM.txt.gz |
174.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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