|
| Status |
Public on Feb 01, 2011 |
| Title |
Hoxa9-ER on day 0, rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Murine bone marrow transduced with epitope tagged forms of Hoxa9 on day 0
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Hoxa9-ER cells
gender: female
age: 6-8 week old
strain: C57BL/6
cell line: murine bone marrow transduced with epitope tagged forms of Hoxa9
time point: day 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner.
|
| Description |
Gene expression data from murine cell line transduced with epitope tagged forms of Hoxa9 day 0
|
| Data processing |
The data were analyzed with robust multi-array average (RMA) normalization using default background adjustment and quantile normalization settings.
|
| |
|
| Submission date |
Apr 12, 2010 |
| Last update date |
Sep 13, 2011 |
| Contact name |
Jay Hess |
| E-mail(s) |
jayhess@med.umich.edu
|
| Phone |
734-763-6384
|
| Fax |
734-763-4782
|
| URL |
http://www.pathology.med.umich.edu/faculty/Hess/index.html
|
| Organization name |
University of Michigan
|
| Department |
Pathology
|
| Street address |
1301 Catherine
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE21299 |
Expression data from murine cell line transduced with epitope tagged forms of Hoxa9 |
| GSE33518 |
Identification and characterization of Hoxa9 binding sites in hematopoietic cells |
|
