|
| Status |
Public on Sep 21, 2021 |
| Title |
R1-tassel |
| Sample type |
SRA |
| |
|
| Source name |
RNA from Tassel
|
| Organism |
Zea mays |
| Characteristics |
tissue: R1-tassel
cultivar: B73
|
| Growth protocol |
Maize (Zea mays ssp. mays) ‘B73’ plants were cultivated in a phytotron at 25°C with 65% relative humidity under a 14-h/10-h light/dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the maize tissues using TRIzol reagent (Molecular Research Center) according to the manufacturer’s instructions. Agilent Bioanalyzer 2100 system was used to check the quality of the extracted RNA, and only RNA of high integrity was used for the analysis.
The degradome-seq library was constructed based on a previously described method (Zhai et al., 2014). Degradome-seq was performed on the Illumina HiSeq 2500 platform (Berry Genomics) to produce 50-bp single- end reads.
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Library strategy: degradome-seq
Illumina’s Genome Analyzer Sequencing Control Software and Pipeline Analysis Software
The raw sequences were filtered to remove low quality reads and the ones that passed the quality filter were trimmed to remove the adaptor sequences.
CleaveLand4.pl was used to processed the data
Supplementary_files_format_and_content: txt file, the result of CleaveLand4, degradome density file
|
| |
|
| Submission date |
May 27, 2021 |
| Last update date |
Sep 22, 2021 |
| Contact name |
Lei Gao |
| E-mail(s) |
leigao@szu.edu.cn
|
| Organization name |
shenzhen university
|
| Department |
College of Life Sciences
|
| Street address |
Nanhai Ave 3688
|
| City |
shenzhen |
| State/province |
guangdong |
| ZIP/Postal code |
518060 |
| Country |
China |
| |
|
| Platform ID |
GPL17628 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN19366739 |
| SRA |
SRX11003433 |
