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Sample GSM5355888 Query DataSets for GSM5355888
Status Public on Jul 15, 2021
Title Kidney IRLacz rep3
Sample type SRA
 
Source name Kidney
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: Kidney
Extracted molecule total RNA
Extraction protocol Kidneys were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Agilent 2100 Bioanalyzer was used.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing cDNA quantity and labeling efficiency was checked using Agilent 2100 Bioanalyzer and ABI StepOne Plus Real-Time PCR System, and sequenced using the Illumina HiSeq platform
The filter software soapnuke (v1.5.2) was used for filtering raw reads, and the RNA seq reads gene comparison was carried out by HISAT (hierarchical indexing for shared alignment of scripts) software (v2.0.4).
Stringtie (v1.0.4) was used to reconstruct the transcripts of each sample, then cuffmerge was used to integrate the reconstruction information of all samples, and then cuffcompare was used to compare the integrated transcripts with the reference annotation information to define the new transcripts.
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
 
Submission date Jun 03, 2021
Last update date Jul 15, 2021
Contact name 汉 朱
E-mail(s) zhuhan19901007@126.com
Organization name
Street address 1095 解放大道, 武汉 430030, 中国
City 武汉
State/province 湖北
ZIP/Postal code 435000
Country China
 
Platform ID GPL21273
Series (2)
GSE176096 The Probiotic L. casei Zhang Slows the Progression of Acute and Chronic Kidney Disease (RNA-Seq)
GSE176098 The Probiotic L. casei Zhang Slows the Progression of Acute and Chronic Kidney Disease
Relations
BioSample SAMN19549487
SRA SRX11053942

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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