|
| Status |
Public on May 19, 2011 |
| Title |
Pt_summer_1 |
| Sample type |
RNA |
| |
|
| Source name |
Poplar stem harvested in summer
|
| Organism |
Populus trichocarpa |
| Characteristics |
tissue: Poplar stem (20 cm sections of 10 cm above ground) harvested in summer (August)
age: one-year-old field grown poplar
|
| Treatment protocol |
no treatment; harvested in summer and winter.
|
| Growth protocol |
Poplar (Populus trichocarpa) were grown on field (nursery bed).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Poplar Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from stem tissue of Poplar harvested in summer (August 2005)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 22, 2010 |
| Last update date |
May 19, 2011 |
| Contact name |
Jae-Heung Ko |
| E-mail(s) |
jhko@khu.ac.kr
|
| Phone |
82-31-201-3863
|
| Organization name |
Kyung Hee University
|
| Department |
Plant & Environmental Resources
|
| Street address |
1 Seocheon-dong, Giheung-gu
|
| City |
Yongin |
| State/province |
Gyeonggi |
| ZIP/Postal code |
446-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE21480 |
Novel aspects of transcriptional regulation in the winter survival and maintenance mechanism of perennial woody plants, poplar. |
|
