|
| Status |
Public on Aug 31, 2021 |
| Title |
THP-1 cell line, JCRB0112.1 |
| Sample type |
genomic |
| |
|
| Source name |
THP-1_JCRB0112.1
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: acute monocytic leukemia
cell line: THP-1
|
| Treatment protocol |
None
|
| Growth protocol |
Cell lines were grown according to standard conditions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the AllPrep DNA/RNA Mini Kit (Qiagen 80204).
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
The data analyses can be carried out using the Chromosome Analysis Suite software, ChAS 4.2 (Thermo Fisher Scientific).
|
| |
|
| Submission date |
Jun 23, 2021 |
| Last update date |
Sep 02, 2021 |
| Contact name |
Fumio Kasai |
| E-mail(s) |
fumio.kasai@riken.jp
|
| Organization name |
RIKEN
|
| Street address |
Koyadai 3-1-1
|
| City |
Tsukuba |
| ZIP/Postal code |
3050074 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16131 |
| Series (2) |
| GSE178731 |
THP-1 cell lines: reference data for classification of THP-1 cells [genome] |
| GSE178741 |
THP-1 cell lines: reference data for classification of THP-1 cells |
|
