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| Status |
Public on Aug 31, 2021 |
| Title |
THP-1 cell line, RCB1189 |
| Sample type |
genomic |
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| Source name |
THP-1_RCB1189
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| Organism |
Homo sapiens |
| Characteristics |
disease state: acute monocytic leukemia
cell line: THP-1
|
| Treatment protocol |
None
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| Growth protocol |
Cell lines were grown according to standard conditions.
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the AllPrep DNA/RNA Mini Kit (Qiagen 80204).
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| Label |
biotin
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| Label protocol |
As per manufacturer (Affymetrix)
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| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
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| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
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| Data processing |
The data analyses can be carried out using the Chromosome Analysis Suite software, ChAS 4.2 (Thermo Fisher Scientific).
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| Submission date |
Jun 23, 2021 |
| Last update date |
Sep 02, 2021 |
| Contact name |
Fumio Kasai |
| E-mail(s) |
fumio.kasai@riken.jp
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| Organization name |
RIKEN
|
| Street address |
Koyadai 3-1-1
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| City |
Tsukuba |
| ZIP/Postal code |
3050074 |
| Country |
Japan |
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| Platform ID |
GPL16131 |
| Series (2) |
| GSE178731 |
THP-1 cell lines: reference data for classification of THP-1 cells [genome] |
| GSE178741 |
THP-1 cell lines: reference data for classification of THP-1 cells |
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| Data table header descriptions |
| ID_REF |
|
| VALUE |
LOG2 RATIO |
| WEIGHTED LOG2 RATIO |
|
| SMOOTH SIGNAL |
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| Data table |
| ID_REF |
VALUE |
WEIGHTED LOG2 RATIO |
SMOOTH SIGNAL |
| C-7SARK |
-0.171368 |
0.143874 |
2.182531 |
| C-4WYLN |
0.325516 |
0.202550 |
2.181873 |
| C-3BFNY |
0.282178 |
0.170266 |
2.181422 |
| C-7ONNE |
0.308231 |
0.227874 |
2.180731 |
| C-6HYCN |
0.479298 |
0.220908 |
2.181248 |
| C-7SCGC |
0.107211 |
0.276409 |
2.180701 |
| C-7RZQQ |
0.238632 |
0.241937 |
2.180153 |
| C-7RZWZ |
0.208469 |
0.294352 |
2.180614 |
| C-7SEBI |
0.172176 |
0.030984 |
2.180588 |
| C-7RZIL |
-0.072699 |
0.079415 |
2.182018 |
| C-4NQTX |
-0.120895 |
0.046289 |
2.182995 |
| C-7SDKW |
0.268082 |
0.089975 |
2.181942 |
| S-3WRNV |
nan |
nan |
nan |
| C-7RXVK |
-0.257399 |
-0.149189 |
2.181633 |
| C-4LHKS |
0.358987 |
-0.110847 |
2.181161 |
| C-7RWHE |
-0.375429 |
-0.133765 |
2.181060 |
| C-7SBEJ |
-0.221096 |
-0.101148 |
2.180895 |
| C-7RYJO |
0.088802 |
0.118120 |
2.180137 |
| C-7SEGS |
0.158362 |
0.144887 |
2.179586 |
| C-4LLXK |
0.232353 |
0.139935 |
2.174773 |
Total number of rows: 2749693
Table truncated, full table size 95920 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM5396659_RCB1189_CytoScanHD_Array_.CEL.gz |
28.5 Mb |
(ftp)(http) |
CEL |
| GSM5396659_THP-1_RCB1189_CytoScanHD_Array_.cyhd.cychp.gz |
96.1 Mb |
(ftp)(http) |
CYCHP |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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