|
| Status |
Public on Jun 14, 2010 |
| Title |
no albendazole exposure, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Adult C. elegans exposed to 1.5% v/v DMSO for 4 hrs
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: whole nematode
genotype: ben-1(e1880)III
culture method: liquid culture
age: 70hrs from L1 (20oC)
treatment: 1.5% v/v DMSO for 4 hrs
|
| Treatment protocol |
70 hr CB3474 cultures were exposed to 300 µg/ml ABZ (diluted from 20mg/ml ABZ in DMSO stock) or DMSO only for four hours, then were harvested by sucrose flotation
|
| Growth protocol |
Synchronised CB3474 L1 (approx. 10000 per experimental condition) were grown in liquid culture at 20oC, 240 rpm for 70 hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip C. elegans Genome Array. GeneChips were washed and stained according to manufacturer supplied protocols.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GCS3000 scanner
|
| Description |
Gene expression data from adult C. elegans exposed to 1.5% v/v DMSO for 4 hrs
|
| Data processing |
The data were analyzed with R and Bioconductor packages (e.g. limma) using RMA as the normalization method.
|
| |
|
| Submission date |
May 07, 2010 |
| Last update date |
May 09, 2010 |
| Contact name |
Steven Thomas Laing |
| E-mail(s) |
steven.t.laing@googlemail.com
|
| Organization name |
University of Glasgow
|
| Department |
Veterinary Medicine
|
| Lab |
Gilleard lab (Henry Wellcome Building)
|
| Street address |
464 Bearsden Road
|
| City |
Glasgow |
| ZIP/Postal code |
G61 1QH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE21747 |
Expression data from Caenorhabditis elegans exposed to albendazole |
|
