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Sample GSM542912 Query DataSets for GSM542912
Status Public on Jun 01, 2010
Title Immature oligodendrocytes d0_Rep3
Sample type RNA
 
Source name Immature oligodendrocytes d0_Rep3
Organism Mus musculus
Characteristics strain: C57BL/6J
genotype/variation: CNP-Cre
cell type: oligodendrocytes
time point: D0
differentiation status: Immature
Treatment protocol No treatment but different time points were analyzed.
Growth protocol Preparation of mouse oligodendrocyte primary cultures were performed as described previously (Simons et al., 2000). After shaking, cells were plated in Dulbecco's modified Eagle's medium (DMEM) with B27 supplement, 1% horse serum, L-thyroxine, tri-iodo-thyronine, glutamine and pyruvate on poly-L-lysinecoated dishes or glass coverslips.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions.
Label Cy3
Label protocol Refer to Agilent miRNA labeling protocol
 
Hybridization protocol Refer to Agilent miRNA hybridisation protocol
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B). Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
Description Immature oligodendrocytes d0_Rep3
Data processing Data were processed using alogorithms specified by the Agilent Feature Extraction software (version A.8.5.1.1). Subsequent statistical analysis (detection of candidates using linear models) of data were carried out using R.
 
Submission date May 12, 2010
Last update date Jun 01, 2010
Contact name Gabriela Salinas
E-mail(s) Gabriela.Salinas-Riester@medizin.uni-goettingen.de
Organization name Universitaetsmedizin Goettingen
Department Department of Pathology
Lab NGS Integrative Genomics
Street address Kreuzbergring 57
City Goettingen
State/province Lower-Saxony
ZIP/Postal code 37075
Country Germany
 
Platform ID GPL10384
Series (2)
GSE21798 Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster (miRNA Agilent Part)
GSE21801 Control of oligodendroglial cell number by the miR-17~92 family of miRNA cluster

Data table header descriptions
ID_REF
VALUE processed Cy3 signal intensity

Data table
ID_REF VALUE
1 2.219006e+002
2 2.319437e+000
3 2.887488e+001
4 3.209668e+001
5 -1.707441e+000
6 1.462702e+001
7 1.221685e+003
9 -1.181601e+000
10 2.056856e+000
11 -2.509614e+000
12 -9.414194e-002
13 9.166560e+001
14 6.536022e-001
15 4.780895e+000
16 1.964602e+002
18 4.912701e+000
19 -4.117337e-001
20 2.609491e+000
21 3.118357e+000
22 7.854467e+000

Total number of rows: 13715

Table truncated, full table size 263 Kbytes.




Supplementary file Size Download File type/resource
GSM542912.txt.gz 1.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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