|
| Status |
Public on May 19, 2011 |
| Title |
WT SVZ tissue |
| Sample type |
RNA |
| |
|
| Source name |
WT SVZ tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain subventricular zone (SVZ)
genotype/variation: wild-type
age: Post natal day 8
|
| Growth protocol |
RNA was isolated from 300um fresh brain sections at the level of the SVZ and SVZ was microdissected and used for RNA extraction using Trizol (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
1ug of total RNA was reverse-transcribed using oligo-dT primers (Superarray) and reverse transcriptase (Superarray) to perform cDNA. Then cRNA synthesis was performed using the total cDNA reaction in the presence of 10 mM of Biotinylated-UTP (Perlin Elmer) and RNA polymerase according to the instructions for the Gene
|
| Label |
Biotin UTP
|
| Label protocol |
cRNA synthesis was performed using the total cDNA reaction in the presence of 10 mM of Biotinylated-UTP (Perlin Elmer) and RNA polymerase according to the instructions for the Gene
|
| |
|
| Hybridization protocol |
1ug of cRNA was used for hybridization with the Genearray. Incubation was performed for 12hrs at 60C shaking at 500rpm.
|
| Scan protocol |
The arrays were exposed at different time points starting for 1 min and maximum of 10 min to have the largest possible dynamic range for the individual signals and images acquire using x-ray film. X-ray film were scanned and save as TIFF file format.
|
| Data processing |
The raw data were re-scaled to account for the differences in individual hybridization intensities and ratios of CNP-hEGFR vs. WT were calculated after values were normalizing for each gene.
|
| |
|
| Submission date |
May 19, 2010 |
| Last update date |
May 19, 2011 |
| Contact name |
Vittorio Gallo |
| E-mail(s) |
vgallo@cnmcresearch.org
|
| Phone |
2024764996
|
| Fax |
2024764988
|
| Organization name |
CNMC
|
| Department |
Center for Neuroscience
|
| Street address |
111 Michigan Av. NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20010 |
| Country |
USA |
| |
|
| Platform ID |
GPL10440 |
| Series (1) |
| GSE21913 |
Gene expression profile of the SVZ of the postnatal mouse brain |
|
