|
| Status |
Public on Nov 01, 2010 |
| Title |
Non-affected sibling |
| Sample type |
genomic |
| |
|
| Source name |
Peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
tissue: Peripheral blood
histological type: Peripheral blood
disease state: healthy
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA from peripheral blood was performed by standard phenol-chlorophorm method
|
| Label |
Cy3
|
| Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37ºC using amplification master mix (WG-MSM) and primer/neutralization mix (WG-MA2). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FMS), precipitate
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48ºC, single-base extension and allele-specific staining was performed (XC1, XC2, TEM, STM, ATM, XC3, PB1) on a Teflow chamber rack system.
|
| Scan protocol |
After allele-specific staining BeadChip arrays were coated with PB1/XC4/ethanol , dried for 1 hour and scanned on a BeadArray Reader (Illumina).
|
| Description |
Genomic DNA extracted from peripheral blood
|
| Data processing |
Image data was analyzed using Beadstudio 3.1.3 with GT module 3.2.33 (Illumina).
|
| |
|
| Submission date |
May 21, 2010 |
| Last update date |
May 21, 2010 |
| Contact name |
Victor Martinez-Glez |
| E-mail(s) |
vmartinezg.hulp@salud.madrid.org
|
| Organization name |
HULP
|
| Department |
INGEMM
|
| Street address |
Paseo la Castellana 261
|
| City |
Madrid |
| ZIP/Postal code |
28046 |
| Country |
Spain |
| |
|
| Platform ID |
GPL8887 |
| Series (1) |
| GSE21958 |
Homozygosity mapping in a consanguineous family with osteogenesis imperfecta (OI) |
|
