|
Status |
Public on Jun 25, 2010 |
Title |
Ago2ko_ES_rep2 |
Sample type |
SRA |
|
|
Source name |
embryonic stem cells
|
Organism |
Mus musculus |
Characteristics |
genotype: Ago2 -/- cell type: embryonic stem cells
|
Treatment protocol |
100nM 4-OH-tamoxifen for 4-9 days for the Drosha experiment
|
Growth protocol |
Standard tissue culture practice
|
Extracted molecule |
total RNA |
Extraction protocol |
Global 5`-phosphate-dependent RACE in WT, Ago2-KO and Drosha-excised mouse ES cells and human 293S cells
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
other |
Instrument model |
Illumina Genome Analyzer II |
|
|
Description |
5`-phosphorylated poly(A) RNA
|
Data processing |
n/a
|
|
|
Submission date |
May 24, 2010 |
Last update date |
Mar 04, 2023 |
Contact name |
Fedor Karginov |
E-mail(s) |
karginov@ucr.edu
|
Organization name |
UC Riverside
|
Department |
MCSB
|
Street address |
900 University Ave
|
City |
Riverside |
State/province |
California |
ZIP/Postal code |
92521 |
Country |
USA |
|
|
Platform ID |
GPL9250 |
Series (1) |
GSE21975 |
Diverse endonucleolytic cleavage sites in the mammalian transcriptome depend upon microRNAs, Drosha, and additional nucleases |
|
Relations |
SRA |
SRX021453 |
BioSample |
SAMN00014153 |