|
| Status |
Public on Jun 07, 2023 |
| Title |
Fmo5(-/-) KO [20183236L] |
| Sample type |
SRA |
| |
|
| Source name |
Liver_Fmo5(-/-) KO
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
strain background: C57BL/6
genotype: Fmo5(-/-)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Livers were removed, flash frozen on dry ice.
Samples were sent to LGC Genomics GmbH (Germany) for RNA extraction (GEN-9300-840), mRNA library (GEN27009) and NextSeq 500 (1x75bp) 400M single reads (GEN27222)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Demultiplexing of all libraries for each sequencing lane using Illumina bcl2fastq2.17.1.14 software (RAW). Clipping of sequencing adapter remnants from all raw reads (AdapterClipped).
Creation of FastQC report for all FASTQ files.
Supplementary_files_format_and_content: *ReadsPerGene.out.tab; read_counts.xlsx; koVswt.csv
|
| |
|
| Submission date |
Jul 21, 2021 |
| Last update date |
Jun 07, 2023 |
| Contact name |
Elizabeth Shephard |
| E-mail(s) |
e.shephard@ucl.ac.uk
|
| Organization name |
University College London
|
| Street address |
Gower Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE180575 |
Fmo5(-/-) mouse: liver transcriptomics |
|
| Relations |
| BioSample |
SAMN20342638 |
| SRA |
SRX11515468 |
