|
| Status |
Public on Nov 16, 2022 |
| Title |
RPE WT rep3 [160121_OPTIMUS_C7NWYANXX.7.11716130] |
| Sample type |
SRA |
| |
|
| Source name |
RPE WT
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Retinal pigment epithelium
age: E11.5
genotype: Wild Type
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RPE were dissected out, stored in RNAlater and total RNA were isolated using Rneasy plus mini kit from Qiagen.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
160121_OPTIMUS_C7NWYANXX.7.11716130
|
| Data processing |
STAR 2.4.2a aligned to GRChm38
HTSeq 0.6.1p1 with gencode.vM8.primary_assembly.annotation.gtf
Genome_build: GRChm38
Supplementary_files_format_and_content: counts
|
| |
|
| Submission date |
Jul 22, 2021 |
| Last update date |
Nov 16, 2022 |
| Contact name |
David McGaughey |
| E-mail(s) |
mcgaugheyd@mail.nih.gov
|
| Organization name |
NIH
|
| Department |
NEI
|
| Street address |
10 Center Dr
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE180641 |
Zfp503/Nlz2 is Required for RPE Differentiation and Optic Fissure Closure |
|
| Relations |
| BioSample |
SAMN20349936 |
| SRA |
SRX11522709 |
