|
Status |
Public on Dec 02, 2022 |
Title |
CKO_2 |
Sample type |
SRA |
|
|
Source name |
retina
|
Organism |
Mus musculus |
Characteristics |
genotype: Mettl3 knockout tissue: Retina age: Postnatal day 7
|
Treatment protocol |
N/A
|
Growth protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
TRIzol NEBNext UltraTM RNA library Prep Kit for Illumina following manufacturer's protocol.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
CK_2
|
Data processing |
Basecalls performed using CASAVA. Clean reads were aligned to the mouse reference genome (mm10) using Hisat2. featureCounts was used to count the reads numbers mapped to each gene. Genome_build: mm10 (GRCm38) Supplementary_files_format_and_content: gene_count.txt: Tab-delimited text file includes read counts for each gene.
|
|
|
Submission date |
Jul 25, 2021 |
Last update date |
Dec 02, 2022 |
Contact name |
SHUYI CHEN |
E-mail(s) |
chenshy23@mail.sysu.edu.cn
|
Organization name |
Zhongshan Ophthalmic Center, SYSU
|
Department |
State Key Laboratory of Ophthalmology
|
Street address |
54 S. Xianlie Rd
|
City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
510060 |
Country |
China |
|
|
Platform ID |
GPL13112 |
Series (2) |
GSE180813 |
Mettl3 regulates late-stage retinogenesis |
GSE180815 |
Mettl3 regulates late-stage retinogenesis and mouse retina m6A epitranscriptome |
|
Relations |
BioSample |
SAMN20394124 |
SRA |
SRX11549607 |