|
| Status |
Public on Dec 01, 2010 |
| Title |
sciP overexpression - rep1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
wt + empty vector - xylose
|
| Organism |
Caulobacter vibrioides CB15 |
| Characteristics |
strain/condition: wild type + empty vector in xylose
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy
|
| Label |
Cy3
|
| Label protocol |
standard Cy3-dCTP incorporation
|
| |
|
| Channel 2 |
| Source name |
sciP overexpression - xylose
|
| Organism |
Caulobacter vibrioides CB15 |
| Characteristics |
strain/condition: wild type + plasmid pJS14-Pxyl-sciP in xylose
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy
|
| Label |
Cy5
|
| Label protocol |
standard Cy5-dCTP incorporation
|
| |
|
| |
| Hybridization protocol |
Agilent protocol
|
| Scan protocol |
Agilent protocol
|
| Description |
N/A
|
| Data processing |
Arrays were scanned using an Agilent scanner. Agilent output files were then opened using the command agferead in Matlab 7.9 and the log10 values extracted with no additional processing or filtering.
|
| |
|
| Submission date |
May 31, 2010 |
| Last update date |
Jun 02, 2010 |
| Contact name |
Michael Laub |
| Organization name |
MIT
|
| Street address |
31 Ames St.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL10469 |
| Series (1) |
|
