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Sample GSM548597 Query DataSets for GSM548597
Status Public on Jul 19, 2010
Title 140_testes_total
Sample type SRA
 
Source name testes
Organism Drosophila virilis
Characteristics tissue: testes
strain: 140
Extracted molecule total RNA
Extraction protocol RNA was size selected (19-30nt) or small RNA-protein complexes were immunoprecipitated from extracts using specific polyclonal antibodies. These were then ligated to linkers at the 3' and 5' end using T4 RNA ligase, then PCR amplified and gel purified.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina Genome Analyzer
 
Description 19-30nt RNA isolated from testes of strain 140
processed_file_build: droVir3
Data processing Sequenced reads were clipped to remove 3' adapter, collapsed to obtain multiplicity, then mapped to relevant genome with no mismatches. Annotations were made based on genomic co-ordinates after mapping. Mapping count refers to number of times a sequence is mapped to the genome.
 
Submission date Jun 01, 2010
Last update date May 15, 2019
Contact name Nikolay V Rozhkov
E-mail(s) nik.rozhkov@gmail.com, rozhkov@cshl.edu
Organization name CSHL
Lab Hannon
Street address 1 Bungtown Rd.
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
 
Platform ID GPL10472
Series (1)
GSE22067 Small RNA-based silencing strategies for transposons in the process of invading Drosophila species
Relations
SRA SRX023738
BioSample SAMN00017171

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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